Table 5. . Corneal endothelial cell culture media.

Media type	Study	Basal media	Supplement	Special supplement	Ref.
Proliferation media	Parekh et al.	Ham's F-12 and M199	5% FBS, 20 μg/ml AA, 1% ITS, 10 ng/ml FGF2, 1% penicillin-streptomycin	10 μM ROCK inhibitor (Y-27632)	[45]
	Peh et al. Wahlig et al.	Ham's F-12 and M199	5% FBS, 20 μg/ml AA, 1% ITS, 10 ng/ml FBF2, 1% antibiotic/antimycotic	10 μM ROCK inhibitor (Y-27632)	[11,55,72,73]
	Okumura et al. Kinoshita et al.	Opti-MEM-I	8% FBS, 5 ng/ml EGF, 20 μg/ml AA, 200 mg/l calcium chloride, 0.08% chondroitin sulfate, 50 μg/ml gentamicin	10 μM ROCK inhibitor (Y-27632)	[12,30]
	Bartakova et al.	Opti-MEM-I	8% FBS, 0.5 mM AA, 200 mg/l calcium chloride, 5 ng/ml EGF, 50 μg/ml gentamicin, 1% antibiotic/antimycotic	100 μg/ml bovine pituitary extract, 20 ng/ml NGF	[57]
Maintenance media	Bartakova et al.	Human endothelial-SFM	4% FBS, 50 μg/ml gentamicin, 1% antibiotic/antimycotic		[57]
	Peh et al. Wahlig et al.	Human endothelial-SFM	5% FBS, 1% antibiotic/antimycotic		[72,73]

AA: Ascorbic acid; FBS: Fetal bovine serum; SFM: Serum-free media.