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. 2021 Sep 2;12:666449. doi: 10.3389/fphys.2021.666449

Figure 3.

Figure 3

Effects of HLJDD on macrophage polarization in ApoE−/− mice with AS. (A) Representative immunohistochemical staining showed the expression of inducible nitric oxide synthase (iNOS) in carotid sections. Magnification, ×200; scale bar=200μm. (B) Representative immunohistochemical staining showed the expression of Arg-1 in carotid sections. Magnification, ×200; scale bar=200μm. (C) The average optical density of iNOS in carotid sections was semi quantitatively analyzed by Image Pro Plus 6.0 software. Values are expressed as the mean±SD (n=5; ##p<0.01 vs. the control group; *p<0.05 and **p<0.01 vs. the model group; one-way ANOVA). (D) The average density of Arg-1 in carotid sections was semi quantitatively analyzed by Image Pro Plus 6.0 software. Values are expressed as the mean±SD (n=5; *p<0.05 and **p<0.01 vs. the model group; one-way ANOVA). (E) The mRNA expression of iNOS and CD86 in carotid tissues were detected by real-time PCR (RT-PCR; n=5; ##p<0.01 vs. the control group; *p<0.05 and **p<0.01 vs. the control group; one-way ANOVA). (F) The mRNA expression of Arg-1 and CD163 in carotid tissues were detected by RT-PCR. Values are expressed as the mean±SD (n=5; ##p<0.01 vs. the model group; *p<0.05 and **p<0.01 vs. the model group; one-way ANOVA).