FIG 2.
IFN-γ is not essential for MyD88-dependent protection against Brucella infection. (A) WT and MyD88−/− mice (n = 4) were treated with anti-IFN-γ and infected in both rear footpads with 1 × 105 B. melitensis 16M. Mice were euthanized at day 7, and joint CFU numbers were enumerated. (B) WT and MyD88−/− mice (n = 5) were treated with anti-IFN-γ and infected i.p. with 1 × 105 B. melitensis 16M. Survival was monitored over time. (C) WT and MyD88−/− mice (n = 5) were treated with anti-IFN-γ and infected i.p. with 1 × 103 B. melitensis 16M. CFU levels were measured in spleen and liver 16 days postinfection. Error bars depict SD from the mean. Data in panel A are from one experiment, while the data in panels B and C are representative of 2 independent experiments. *, P < 0.05 compared to WT, anti-IFN-γ-treated mice.