FIG 3.

Comparison of RF uptake by wild-type B. burgdorferi and the 2D7 mutant. B. burgdorferi was grown to late log phase and then incubated at 37°C in medium supplemented with labeled RF (10 or 100 μM) for 12 h. Cell pellets were washed twice with BSK-II medium and then frozen. Labeled RF (A), FMN (B), and FAD (C) concentrations were determined by LC-MS. Each data point represents a biological replicate (n = 6) from three independent experiments. Bars represent the averages of the displayed data points, and error bars indicate SD. One-way ANOVA with Sidak’s post hoc test was used for multiple comparisons. ns, not significant; **, P < 0.01; ***, P < 0.001.