FIG 5.

Involvement of Dectin-2 and CARD9 in actin polymerization. (A) BM-DCs from WT mice were pretreated with cytochalasin D (CyD) for 30 min. These BM-DCs were cultured with FITC-labeled C. neoformans Cap67 for 2 h. Phagocytosed cells were analyzed using flow cytometry. (B, C, D) BM-DCs prepared from WT and Dectin-2KO and CARD9KO mice were cultured with FITC-labeled C. neoformans Cap67 for 30 min. BM-DCs were spun down onto a glass slide, stained with Acti-stain 555 phalloidin, and observed under a confocal laser scanning microscope. BM-DCs with strong actin polymerization (red) at sites where they were bound to C. neoformans (green) were counted, and the percentages of all cells with this characteristic were calculated. Each column represents the mean ± SD of triplicate cultures. *, P < 0.05.