The ΔkatA mutant exhibits a biofilm biomass defect on silicone catheters. (A) Representative images of crystal violet-stained 15-mm silicone Foley catheter segments after 20 h of incubation with the WT, the ΔkatA mutant, the ΔkatA mutant plus bovine catalase, or plain LB (blank). (B) Biomasses of 20-h WT (gray), ΔkatA mutant (white), or ΔkatA mutant plus bovine catalase (light gray) catheter biofilms as assessed by crystal violet staining at an OD570. Error bars represent means and SDs from at least 3 independent experiments with at least 3 technical replicates each. **, P < 0.01; ***, P < 0.001 (by one-way ANOVA with Tukey’s test for multiple comparisons).