Table 1.
Statistically significant AA substitutions in PTM sites
| PTM annotation | From AA | To AA | # sub. w/ annotation | # sub. w/o annotation | # variants FDR q-value | AF FDR q-value | Trenda |
|---|---|---|---|---|---|---|---|
| Acetylation | A | A | 212 | 1 54 569 | 1.94E-02 | E | |
| A | T | 51 | 91 640 | 4.15E-10 | 1.27E-02 | D/D | |
| A | V | 122 | 84 602 | 3.16E-02 | D | ||
| K | K | 398 | 48 774 | 6.89E-03 | E | ||
| K | N | 128 | 22 414 | 2.35E-02 | D | ||
| K | Q | 63 | 10 543 | 4.75E-02 | D | ||
| Disulfide bond | C | C | 2014 | 25 117 | 9.70E-32 | E | |
| C | F | 311 | 5700 | 6.68E-03 | D | ||
| C | G | 238 | 4429 | 8.13E-03 | 2.62E-02 | D/D | |
| C | R | 648 | 11 634 | 3.93E-04 | 6.99E-05 | D/D | |
| C | S | 412 | 8104 | 2.56E-06 | 1.01E-02 | D/D | |
| C | W | 175 | 3205 | 4.18E-02 | D | ||
| C | Y | 870 | 16 183 | 6.68E-08 | 5.26E-05 | D/D | |
| Hydroxylation | K | K | 105 | 49 091 | 7.65E-05 | E | |
| Methylation | K | K | 54 | 49 140 | 2.36E-03 | E | |
| R | R | 154 | 86 468 | 3.05E-02 | E | ||
| N-linked Glycosylation | N | D | 657 | 16 080 | 1.79E-02 | D | |
| N | H | 274 | 7345 | 6.70E-03 | D | ||
| N | N | 2441 | 51 886 | 2.25E-02 | E | ||
| O-linked Glycosylation | S | S | 82 | 1 48 408 | 1.74E-02 | E | |
| T | T | 105 | 1 42 197 | 1.44E-02 | E | ||
| Phosphorylation | S | A | 285 | 9066 | 4.08E-02 | 2.68E-03 | D/D |
| S | F | 1054 | 25 354 | 4.47E-04 | E | ||
| S | L | 1176 | 30 503 | 3.41E-02 | D | ||
| S | G | 706 | 21 817 | 6.68E-03 | D | ||
| S | S | 5403 | 1 43 087 | 1.96E-02 | E | ||
| S | T | 669 | 19 274 | 3.39E-04 | D | ||
| T | T | 1471 | 1 40 831 | 3.41E-02 | D | ||
| Y | H | 85 | 15 654 | 1.38E-02 | D | ||
| Y | Y | 454 | 50 960 | 3.06E-06 | E | ||
| Succinylation | K | E | 66 | 28 051 | 2.08E-02 | D | |
| K | K | 106 | 49 066 | 2.08E-02 | D | ||
| K | R | 86 | 35 506 | 2.08E-02 | E | ||
| Ubiquitination | K | E | 4091 | 24 020 | 3.40E-10 | D | |
| K | I | 324 | 2060 | 1.47E-03 | D | ||
| K | K | 7536 | 41 630 | 1.67E-06 | 3.47E-07 | E/D | |
| K | M | 422 | 2752 | 4.78E-02 | 5.41E-03 | D/D | |
| K | N | 2980 | 19 562 | 3.29E-10 | 7.44E-09 | D/D | |
| K | Q | 1477 | 9129 | 4.78E-02 | D | ||
| K | R | 5420 | 30 168 | 1.30E-03 | 2.47E-09 | E/D | |
| K | T | 1589 | 9119 | 1.40E-07 | D |
aTrends of significant differences between annotated and unannotated residues. Symbols indicate enrichment (E) or depletion (D). When both statistical tests, based on the number of variants (left) and allele frequency (right), are significant (q-value < 0.05), then two distinct symbols are shown. Synonymous substitutions that maintain the same AA are shown in bold.