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. 2021 Sep 13;31(17):3894–3904.e5. doi: 10.1016/j.cub.2021.06.002

Figure 4.

Figure 4

Quantification of experimental and control female immobility during tremulation and during the rest of the courtship in pairs with OrR males

Data for pairs including control females carrying either 86D09-Gal4 or 73D10-Gal4 (each Gal4 targets different subsets of neurons in the legs) or either the UAS-TNTE, UAS-TRPA1 or one of the UAS-RNAi lines and for pairs including experimental females carrying a combination of both a Gal4 and an upstream activating sequence (UAS) and pairs including a dpiezoKO/Df(2L)Exel7034 female. In (A) and (B), ethograms were constructed from analysis of 30, 18, 19, 18, 26, 30, 23, 10, 12, 13, and 10 pairs (in the order illustrated on the graphs).

(A) The percentage of time where females were immobile while the male was tremulating is significantly lower only for pairs that included a female 86D09-Gal4>UAS-TNTE, 86D09-Gal4>UAS-nanRNAi, or 86D09-Gal4 > UAS-dpiezoRNAi. dpiezoKO/Df(2L)Exel7034 females behave similarly to 86D09-Gal4>UAS-dpiezoRNAi females.

(B) Same pairs as (A). The percentage of time where females were immobile while the male was not tremulating is 40% lower for 86D09-Gal4 control females compared with 86D09-Gal4>UAS-TNTE females, but the immobility of the UAS-TNTE control line is only 7.5% lower than that of 86D09-Gal4>UAS-TNTE females; all other pairs behaved similarly to all their associated controls. The immobility of dpiezoKO/Df(2L)Exel7034 females is similar to that of 86D09-Gal4 > UAS-dpiezoRNAi females.

(C) The percentage of time where females were immobile while the male tremulated is shown for pairs filmed at high temperature (HT) (31°C), including control females carrying only the 86D09-Gal4 construct (n = 17) or 86D09Gal4 > UAS-TRPA1 females (n = 16). Data are also shown at room temperature (RT) (23°C) for pairs including females 86D09-Gal4>UAS-TRPA1 (n = 6). Female immobility during male tremulation is similarly high in all 3 types of pairs. This is also the case for total female immobility and male tremulation during courtship (Figures S2E and S2F).

(D) The percentage of time where females were immobile while the male abdomen was not tremulating is shown for the same pairs as (C). 86D09-Gal4>UAS-TRPA1 female immobility at high temperature is significantly higher than controls at RT and HT.

See also Figures S2 and S3, Table S1, and Video S2.