REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat FITC-conjugated anti-GFP	AbCam	Cat# ab6662; RRID: AB_305635
mouse anti-NC82	Hybridoma bank	Cat# nc82; RRID: AB_2314866
anti-mouse Cy5	JacksonImmunoResearch	Cat# 715-175-151; RRID: AB_2340820
Experimental models: organisms/strains
Wild Drosophila melanogaster	Collected from Wild (see Experimental model and subject details)	N/A
Drosophila melanogaster Oregon-R	Gift from the Lawrence laboratory, Cambridge	N/A
Drosophila melanogaster dPiezo-Gal4	BDSC 58771 (on chr. II)	FBti0164865
	BDSC 59266 (on chr. III)	FBti0166812
Drosophila melanogaster Nanchung-Gal4	BDSC 24903	FBti0101148
Drosophila melanogaster R86D09-Gal4	BDSC 40459	FBti0139154
Drosophila melanogaster R73D10-Gal4	BDSC 39819	FBti0138087
Drosophila melanogaster R64C04-Gal4	BDSC 39296	FBti0137440
Drosophila melanogaster R46H11-Gal4	BDSC 50284	FBti0136107
Drosophila melanogaster UAS nanchungRNAi	BDSC 53312	FBti0157920
Drosophila melanogaster UAS-trpγRNAi	BDSC 53313	FBti0157921
Drosophila melanogaster UAS-TNTE	BDSC 28837	FBti0038528
Drosophila melanogaster UAS-TRPA1	BDSC 26264	FBti0114502
Drosophila melanogaster UAS-piezoRNAi	NIG-FLY 8486R-1	FBal0267721
Drosophila melanogaster UAS-mCD8-GFP	BDSC 5137	FBti0012685
Drosophila melanogaster PiezoKO	BDSC 58770	FBti0147345
Drosophila melanogaster Df(2L)Exel7034	BDSC 7807	FBab0037912
Software and algorithms
Excel Macro to build ethograms from annotated movies	Described in Fabre et al.8	Movie-to-ethogram.xlsm available at: https://github.com/CarolineFabre/Excel-Macro-for-ethograms-
R algorithm to analyze overlapping courtship behaviors	Described in Fabre et al.8	script_behavior_R available at: https://github.com/CarolineFabre/Script_behaviour.R