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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Laser Photon Rev. 2021 Jun 27;15(8):2100072. doi: 10.1002/lpor.202100072

Figure 5. Imaging of 3D distributed fluorescent particles.

Figure 5.

(a) Reconstruction from pixel back projection algorithm for different depth. (b) Background suppression of (a) through the particle clustering algorithm. Different particle clusters are separated at different depths. The clustered particles are intentionally blurred for a clearer visualization. (c) 3D volumetric view of the reconstructed volume. Each point has a fixed axial span, the same as the depth interval between adjacent slice in the reconstruction. Inset: raw captured image. (d) Maximum intensity projection view of the reconstructed particle clusters in 3D at xy, xz and yz plane. (e) Images captured by a benchtop microscope and stitched for same field of view as (c-d). (f) zoomed-in view of the same selected regions identified by the dashed line in (d) and (e). Scale bar for (a-e): 2 mm; (f): 500 μm.