Fig 5. D-11 increase the anti-biofilm ability of azithromycin.

(A) The biofilm was grown at the indicated conditions and stained with crystal violet. The crystal violet was dissolved in ethanol and measured at a wavelength of 595 nm. (B) The biofilm was grown at the indicated conditions and dispersed by ultrasound. The bacterial count inside of biofilm was determined by plating. The established biofilm was treated with indicated conditions for 24 hours. The bacterial count inside the biofilm (C) or in the supernatant (D) was determined by plating. All the tests were performed in triplicate and all the data are presented as mean ± SEM. **, P < 0.01; ***, P < 0.001; ns, not significant by Student’s t-test.