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. 2021 Sep 3;15(9):e0009743. doi: 10.1371/journal.pntd.0009743

Fig 2. Antigen specific plasmablasts increase following vaccination.

Fig 2

(A) Representative flow cytometry plot of the plasmablast population from one donor on day 0 and 7 post vacation. (B) Summary flow cytometry analysis of the plasmablast population following vaccination for each of the 12 participants on day 0, 7, 10, 15, and 30 post vaccination. (C) Representative ELISPOT of the total, LPS, and CTB specific IgM, IgG, and IgA ASC from one subject on day 7 post vaccination. (D) Summary ELISPOT analysis of lipopolysaccharide (LPS) and cholera toxin B subunit specific IgM, IgG, and IgA antibody secreting cells following vaccination from each of the 12 subjects on day 0, 7, and 30 post vaccination. Dotted line indicates limit of detection for the ELISPOT assay. (E) Isotype distribution of IgM (black) IgG (grey), and IgA (white) of all ASC for each of the 12 subjects. Dotted line indicates limit of detection for the ELISPOT assay. Statistical significance between bracketed samples was determined using a one-way ANOVA. Significance values are indicated by asterisks (P < 0.05 (*); P < 0.005 (**); P < 0.0005 (***); P <0.0001 (****)).