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. 2021 Sep 3;17(9):e1009787. doi: 10.1371/journal.pgen.1009787

Fig 2. CRISPR-generated deletion and frameshift alleles of atlas confirm the gene’s requirement for male fertility.

Fig 2

A) Single-pair fertility assay for males homozygous for the null (Δatlas) allele or heterozygous controls (Δatlas/SM6). Flies homozygous for the deletion had significantly reduced fertility (control fertility: 82.9 ± 4.5; null fertility: 0.3 ± 0.6; two-sample t-test assuming unequal variances, p = 5.4 x 10−18). B) Single-pair fertility assays for males homozygous or heterozygous for three frameshift alleles of atlas generated by imprecise non-homologous end joining at a CRISPR/Cas9 target site just downstream of the start codon: atlas52 (control fertility: 104.7 ± 3.8, mutant fertility: 11.2 ± 4.6; two-sample t-test assuming unequal variances: p = 8 x 10−12), atlas62 (control fertility: 96.2 ± 5.7; mutant fertility: 8.4 ± 2.9; two-sample t-test assuming unequal variances: p = 6.1 x 10−10) and atlas86 (control fertility: 67.5 ± 6.6; mutant fertility: 17.3 ± 5.8; two-sample t-test assuming unequal variances: p = 9.5 x 10−6).