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Staging of condensing spermatid nuclei fixed in paraformaldehyde from atlas-GFP males stained with TO-PRO-3 DNA stain. Atlas-GFP is not detectable in (A) round stage or (B) early canoe stage nuclei. Atlas-GFP is nuclear localized in the late canoe stage (C). When nuclei become fully elongated (D), puncta of Atlas-GFP appear to be removed from the nucleus.
