Table 1.
Main effects of a GLMMPQL analysis examining the effects of treatment, experimental day, mean temperature and temperature change; the variances due to tank (n = 6) and individual (ID) (n = 18) were accounted for in the model as nested random factors
| Body mass change ~ treatment + day + mean temp * temp change, random = list(~1|Tank,~1|ID) | |||||
|---|---|---|---|---|---|
| Value | Std error | DF | t | P | |
| (Intercept) | −14.82 | 6.37 | 122 | −232 | 0.021* |
| Current climate treatment | −10.45 | 2.38 | 3 | −4.38 | 0.021* |
| Warmer climate treatment | −10.93 | 2.57 | 3 | −4.24 | 0.024* |
| Day | −0.32 | 0.007 | 122 | −4.35 | >0.001*** |
| Mean temp | 1.54 | 0.43 | 122 | 3.54 | 0.006** |
| Temp change | 3.28 | 0.78 | 122 | 4.21 | >0.001*** |
| Mean temp: temp change | −0.153 | 0.049 | 122 | −3.11 | >0.002** |
| N:L ~ treatment + day + mean temp + temp change, random = list(~1|Tank,~1|ID) | |||||
| Value | Std error | DF | t | P | |
| Intercept | 0.081 | 0.015 | 99 | 5.10 | >0.001*** |
| Current climate treatment | 0.038 | 0.02 | 3 | 1.79 | 0.171 |
| Warmer climate treatment | 0.055 | 0.02 | 3 | 2.45 | 0.091 |
| Day | 0.000007 | 0.00005 | 99 | 0.13 | 0.89 |
| Mean temp | −0.002 | 0.0009 | 99 | −2.09 | 0.038* |
| Temp change | −0.005 | 0.0018 | 99 | −3.09 | 0.003** |
| PKA ~ treatment + day + mean temp + temp change, random = list(~1|Tank,~1|ID) | |||||
| Value | Std error | DF | t | P | |
| Intercept | 26.38 | 2.87 | 86 | 9.18 | >0.001*** |
| Current climate treatment | −10.29 | 3.73 | 3 | −2.75 | 0.07 |
| Warmer climate treatment | −7.31 | 4.05 | 3 | −1.80 | 0.16 |
| Day | 0.09 | 0.012 | 86 | 7.29 | >0.001*** |
| Mean temp | −0.002 | 0.0009 | 86 | −2.09 | 0.038* |
| Temp change | 0.86 | 0.38 | 86 | 2.26 | 0.026* |
| SKA ~ treatment + day + mean temp + temp change, random = list(~1|Tank,~1|ID) | |||||
| Value | Std error | DF | t | P | |
| Intercept | 28.61 | 3.47 | 94 | 8.23 | >0.001*** |
| Current climate treatment | −6.93 | 4.65 | 3 | −1.48 | 0.23 |
| Warmer climate treatment | −1.51 | 4.99 | 3 | −0.30 | 0.78 |
| Day | 0.03 | 0.013 | 94 | 2.12 | >0.03* |
| Mean temp | −0.14 | 0.21 | 94 | −0.70 | 0.48 |
| Temp change | 0.06 | 0.43 | 94 | 0.13 | 0.89 |
| EKA ~ treatment + day + mean temp + temp change, random = list(~1|Tank,~1|ID) | |||||
| Value | Std error | DF | t | P | |
| Intercept | 44.64 | 12.48 | 86 | 3.57 | >0.006** |
| Current climate treatment | −52.53 | 16.48 | 3 | −3.18 | 0.049* |
| Warmer climate treatment | −64.62 | 17.68 | 3 | −3.65 | 0.035* |
| Day | −0.05 | 0.05 | 86 | −1.08 | 0.28 |
| Mean temp | 1.62 | 0.73 | 86 | 2.21 | 0.029* |
| Temp change | 3.80 | 1.54 | 86 | 2.46 | 0.015* |