Skip to main content
. Author manuscript; available in PMC: 2021 Sep 16.
Published in final edited form as: Oncogene. 2008 Jan 28;27(25):3596–3604. doi: 10.1038/sj.onc.1211016

Figure 2.

Figure 2

Expression of Src in the dorsolateral prostate during progressive stages of prostate cancer development in transgenic adenocarcinoma of mouse prostate (TRAMP) mice. (a) Protein levels of Src and phospho-Src (Tyr 416) by immunoblot analysis. Equal loading of protein was confirmed by stripping the blot and reprobing with β-actin antibody. Western blot analysis was conducted in five animals in each group, and only representative blots are shown. Values on top of bands represent relative densities normalized to β-actin. (b) Immunohistochemical analysis as detailed in ‘Materials and methods’, the protein levels were determined in the dorsolateral prostate of 8-, 16-, and 24-week-old TRAMP mice. Immunostaining data were confirmed in two slides from five animals. Photomicrographs (magnification, × 20 and × 40) represent immunohistochemical staining for Src in TRAMP mice with moderately differentiated carcinoma (24 weeks).