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. 1999 Mar;37(3):538–543. doi: 10.1128/jcm.37.3.538-543.1999

FIG. 3.

FIG. 3

Results of PCR amplification of MBA genes with primers UMS-125 and UMA269′ followed by restriction enzyme analysis with HinfI. Lanes M, molecular weight markers ΦX174 DNA/HaeIII; lanes 1 and 2, U. urealyticum serovar 1 ATCC strain PCR products before and after digestion by HinfI; lanes 3 and 4, U. urealyticum serovar 1 clinical isolate PCR products before and after digestion by HinfI; lanes 5 and 6, U. urealyticum serovar 1 positive clinical specimen PCR products after digestion by HinfI; lanes 7 and 8, U. urealyticum serovar 6 ATCC strain PCR product before and after digestion by HinfI; lane 9, U. urealyticum serovar 6 clinical isolate PCR product after digestion by HinfI; lanes 10 to 13, PCR products of four clinical specimens digested by HinfI. Lanes 10 and 12 were identified as serovar 1, and lanes 11 and 13 were identified as serovar 6.