Results of PCR amplification of MBA genes of all 14 serovars of U. urealyticum with primers UMS-54 (specific for serovar 6) and UMA269′. A positive result is shown by a 369-bp band. Lanes M, molecular weight markers ΦX174 DNA/HinfI; lanes 1 and 2, U. urealyticum serovar 1 ATCC strain and clinical isolate; lanes 3 to 6, U. urealyticum serovars 2 to 5 ATCC strains; lanes 7 and 8, U. urealyticum serovar 6 ATCC strain and clinical isolate; lanes 9 to 16, U. urealyticum serovars 7 to 14 ATCC strains.