Fig. 2.

Interaction of β-adaptin with GluA1 via the KRMK motif. GST pulldown assay showing binding of β-adaptin in brain cytosolic extracts to GST fusion proteins of the cytoplasmic tails of GluA1 (GluA1C) and the indicated KRMK motif mutants. GST and GluA2C served as negative and positive controls for β-adaptin pulldown, respectively. Coomassie staining shows the amount of GST fusion proteins bound to beads in each lane (KD, kilodalton). Quantification of mean β-adaptin signal intensity is shown below as the mean±s.e.m. of n=4 different experiments (a.u., arbitrary units). *P<0.05, **P<0.01 (one-way ANOVA with uncorrected Fisher's LSD).