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. 2021 Sep 6;134(17):jcs258685. doi: 10.1242/jcs.258685

Fig. 2.

Fig. 2.

Amino acid starvation enhances cell salt stress-induced Sec body formation. (A,A′) Immunofluorescence (IF) visualization (A) and quantification (A′) of Sec body formation (marked by Sec16) in cells incubated in SCH84, KRB, KRB supplemented with 5–40 mM amino acids (AA) for 4 h at 26°C, as well as KRB for 4 h followed by addition of 5 mM amino acids for 1 h at 26°C. (B) Quantification of Sec body formation (marked by Sec16) in cells incubated in KRB and SCH150 with or without the SIK inhibitor HG-9-91-01(5 µM). (C) Quantification of Sec body formation (marked by Sec16) in cells incubated in KRB and SCH150 with or without the Src inhibitor dasatinib (Da, 20 µM), the p38 MAPK inhibitor SB203580 (SB, 30 µM), Dorsomorphin (1 µM) and ON123300 (10 µM) for 4 h at 26°C, as well in cells incubated in Schneider's medium (Sch) and Schneider's medium buffer supplemented with or without the AMPK activator AICAR (1 mM). ‘d’ indicates the mean±s.e.m. decrease in Sec body formation when compared to the absence of inhibitors. Scale bars: 10 µm. Errors bars: s.e.m.