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. 2021 Sep 6;134(17):jcs258428. doi: 10.1242/jcs.258428

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Mapping lmn-1 to the mTOR pathway. (A) Average length of N2 young adult worms fed with RNAi for either lmn-1, rheb-1, both lmn-1 and rheb-1 or EV. n(N2)=494. (B) Average length of eat-2 young adult worms fed with RNAi for either lmn-1, rheb-1, both lmn-1 and rheb-1 or EV. n(eat-2)=680. (C) Average length of N2, eat-2, raga-1 or eat-2;raga-1 young adult worms fed with lmn-1 (RNAi) or EV. n(N2)=70, n(raga-1)=92, n(eat-2)=73, n(eat-2;raga-1)=45. (D) Average length of N2, eat-2, daf-2 or eat-2;daf-2 young adult worms fed with lmn-1 (RNAi) or EV. n(N2)=96, n(eat-2)=92, n(daf-2)=72, n(eat-2;daf-2)=51. Experimental conditions for daf-2 and eat-2;daf-2 differed from those for N2 and eat-2 (dashed line; see Materials and Methods for details). (E) Average length of N2, eat-2, daf-16, akt-1, eat-2;daf-16 or eat-2;akt-1 young adult worms fed with lmn-1 (RNAi) or EV. n(N2)=80, n(daf-16)=95, n(akt-1)=115, n(eat-2)=72, n(eat-2;daf-16)=82, n(eat-2;akt-1)=97. (F) Average length of N2, eat-2 or aak-2 young adult worms fed with lmn-1 (RNAi) or EV. n(N2)=50, n(aak-2)=43, n(eat-2)=42, n(eat-2;aak-2)=57. Error bars in all graphs represent mean±s.e.m. *P<0.01; **P<0.001; ***P<0.0001; ns, not significant (two-tailed unpaired t-test).