| Antibody |
Podoplanin (T1-α) monoclonal PE-Cyanine7 |
eBioscience/Thermo Fisher Scientific |
RRID:AB_2573459
|
Host: Syrian hamster0.125 µg/100 µl |
| Antibody |
CD31 (PECAM-1) FITC |
eBioscience/Thermo Fisher Scientific |
RRID:AB_465012
|
Host: rat1 µg/100 µl |
| Antibody |
CD326 (EpCAM) monoclonal APC-eFluor780 |
eBioscience/Thermo Fisher Scientific |
RRID:AB_2573986
|
Host: rat0.125 µg/100 µl |
| Antibody |
CD24 monoclonal, APC |
eBioscience/Thermo Fisher Scientific |
RRID:AB_10870773
|
Host: rat0.06 µg/100 µl |
| Antibody |
CD45 monoclonal (30-F11), PE |
eBioscience/Thermo fisher Scientific |
RRID:AB_465668
|
Host: rat0.125 µg/100 µl |
| Antibody |
TER-119 monoclonal, biotin |
eBioscience/Thermo fisher Scientific |
RRID:AB_466796
|
Host: rat1:100 |
| Antibody |
CD16/32, clone 2.4G2, biotin |
BD Biosciences |
RRID:AB_394658
|
Host: rat1:100 |
| Antibody |
CD104 [346-11A] biotin |
BioLegend |
RRID:AB_961034
|
Host: rat1:100 |
| Antibody |
CD31 [MEC13.3] biotin |
BioLegend |
RRID:AB_312910
|
Host: rat1:100 |
| Antibody |
CD45, biotin |
eBioscience/Thermo Fisher Scientific |
RRID:AB_466446
|
Host: rat1:100 |
| Antibody |
Ki-67/MKI67 polyclonal |
Novus Biologicals |
RRID:AB_10001977
|
Host: rabbit1:100 |
| Antibody |
Podoplanin polyclonal |
R&D Systems |
RRID:AB_2268062
|
Host: goat15 µg/ml |
| Antibody |
Phospho-histone H2A.X (Ser139) |
Cell Signaling Technology |
RRID:AB_2118010
|
Host: mouse1:100 |
| Antibody |
Pro-surfactant protein C (SFTP-C) |
Millipore Sigma |
RRID:AB_91588
|
Host: rabbit1:1000 |
| Antibody |
Platelet-derived growth factor receptor-α |
R&D Systems |
RRID:AB_2236897
|
Host: mouse1:50 |
| Antibody |
α-smooth muscle actin |
American Research Product |
RRID:AB_1540376
|
Host: mouse1:500 |
| Antibody |
Goat anti-rabbit IgG secondary antibody-Alexa Fluor 594 |
Thermo Fisher Scientific |
RRID:AB_2556545
|
Host: goat2 µg/ml |
| Antibody |
Donkey anti-goat IgG secondary antibody-Alexa Fluor 594 |
Thermo Fisher Scientific |
RRID:AB_2534105
|
Host: donkey2 µg/ml |
| Antibody |
Donkey anti-mouse IgG secondary antibody-Alexa Fluor 594 |
Thermo Fisher Scientific |
RRID:AB_2556543
|
Host: donkey2 µg/ml |
| Antibody |
Donkey anti-rabbit IgG secondary antibody-Alexa Fluor 488 |
Thermo Fisher Scientific |
RRID:AB_2556546
|
Host: donkey2 µg/ml |
| Antibody |
Anti-Biotin MicroBeads UltraPure |
Miltenyi Biotec |
AB_2811216 |
Host: mouse |
| Chemical compound, drug |
Fluorescent cell staining reagents |
Fixable Viability Dye eFluor 450 |
eBioscience/Thermo Fisher Scientific |
|
| Chemical compound, drug |
Fluorescent cell staining reagents |
Phalloidin-iFluor 594 Reagent -CytoPainter |
Abcam |
|
| Chemical compound, drug |
Fluorescent cell staining reagents |
Hoechst 33342trihydrochloride trihydrate |
Thermo Fisher Scientific |
|
| Chemical compound, drug |
Bovine pituitary extract |
Millipore Sigma |
P1476-2.5ML |
MTEC/plus supplement |
| Chemical compound, drug |
Collagenase, type 4 |
Worthington Biochemical |
LS004188 |
L-MSC isolation |
| Chemical compound, drug |
Dispase II |
Millipore Sigma |
4942078001 |
AEC2 isolation |
| Chemical compound, drug |
Matrigel MembraneMatrix GFR |
Corning |
356231 |
Alveolosphere assay |
| Chemical compound, drug |
Insulin-transferrin-selenium-ethanolamine (ITS -X) (100X) |
Thermo Fisher Scientific |
51500056 |
MTEC/plus supplement |
| Chemical compound, drug |
Human insulin soln. |
Millipore Sigma |
I9278-5ML |
MTEC/plus supplement |
| Chemical compound, drug |
EGF recombinantmouse protein |
Thermo Fisher Scientific |
PMG8041 |
MTEC/plus supplement |
| Chemical compound, drug |
STEMRD Y27632ROCK inhibitor |
Thermo Fisher Scientific |
50-175-997 |
MTEC/plus supplement |
| Chemical compound, drug |
All-trans retinoic acid |
Millipore Sigma |
R2625-100MG |
MTEC/plus supplement |
| Chemical compound, drug |
Cholera toxin |
Millipore Sigma |
C8052-5MG |
MTEC/plus supplement |
| Chemical compound, drug |
Recombinant humanTGF-beta1 |
PeproTech |
100-21-2ug |
|
| Chemical compound, drug |
Horseradish peroxidase |
Millipore Sigma |
P8375-25KU |
|
| Chemical compound, drug |
Homovanillic acid,fluorimetric reagent |
Millipore Sigma |
H1252-100MG |
|
| Chemical compound, drug |
Hydrogen peroxide solution, 30% (w/w) |
Millipore Sigma |
H1009-100ML |
|
| Chemical compound, drug |
Oligomycin |
Millipore Sigma |
O4876-5MG |
|
| Chemical compound, drug |
Carbonyl cyanide 4 |
Millipore Sigma |
C2920-10MG |
|
| Chemical compound, drug |
Antimycin A |
Millipore Sigma |
A8674-25MG |
|
| Chemical compound, drug |
2-deoxy D-glucose |
Millipore Sigma |
D6134-5G |
|
| Chemical compound, drug |
Vectashield |
Vector Laboratories |
H-1400-10 |
|
| Chemical compound, drug |
HistoGel; specimenprocessing gel |
Richard-Allan Scientific/Thermo Fisher Scientific |
HG-4000-012 |
|
| Commercial assay or kit |
Pierce BCA ProteinAssay Kit |
Thermo Fisher Scientific |
PI23225 |
|
| Commercial assay or kit |
EZQ Protein Quantitation Kit |
Thermo Fisher Scientific |
R33200 |
|
| Commercial assay or kit |
MidiMACS Starting Kit (LS) |
Miltenyi Biotec |
130-042-301 |
|
| Commercial assay or kit |
Senescence DetectionKit |
Abcam |
Ab65351 |
|
| Commercial assay or kit |
Mouse XL Cytokine Array Kit |
R&D Systems |
ARY028 |
|
| Commercial assay or kit |
ADP/ATP Ratio Kit |
Abcam |
Ab65313 |
|
| Commercial assay or kit |
Novex Colloidal Blue Staining kit |
Invitrogen |
LC6025 |
|
| Strain, strain background (Mus musculus) |
C57BL/6J (2 months) male |
Jackson Laboratory |
IMSR_JAX:000664 |
|
| Strain, strain background (Mus musculus) |
C57BL/6J (18 months) male |
National Institute of Aging |
N/A |
|
| Strain, strain background (Mus musculus) |
C57BL/6J (Nox4 knockout) male |
Dr. Karl-Heinz Krause |
University of Geneva |
|
| Other |
Mouse genotyping service |
Transnetyx, Inc, Cordova, TN |
|
|
| Chemical compound, drug |
Isoflurane |
VetOne |
NDC 13985-528-60 |
Anesthesia agent |
| Other |
Inverted microscope |
Keyence |
SCR_017202 |
|
| Other |
Inverted microscope |
Carl Zeiss |
|
|
| Other |
Nikon A1R confocal microscope |
Nikon |
|
|
| Other |
Microplate reader |
BioTek |
SynergyMx |
|
| Other |
Centrifuge |
Eppendorf |
5702R |
|
| Other |
Flow Cell Sorter |
BD Biosciences |
|
|
| Other |
BD FACS Aria |
BD Biosciences |
SCR_018091 |
|
| Other |
Flow Cell Analysis |
BD Biosciences |
BD LSR II GUAVA EasyCyte |
|
| Other |
Extracellular Flux Analyzer (Seahorse XFe96) |
Agilent |
SCR_019545 |
|
| Other |
LS columns (magnetic separation) |
Miltenyi Biotec |
130-042-401 |
AEC2 purification |
| Other |
Extracellular FluxAnalyzer |
Agilent |
Seahorse XFe96 |
|
| Software, algorithm |
ImageJ |
Wayne Rasband (NIH) |
https//github.com/imagej/imagej1
|
Johannes, 2021
|
| Software, algorithm |
Image Quant |
Cytiva |
Version TL8.1 |
|
| Software, algorithm |
FlowJo |
Tree Star Inc |
|
|
| Software, algorithm |
Nis Elements |
Nikon |
Version 5.0 |
|
| Sequence-based reagent |
Mouse Nox4 forward |
IDT |
ACT TTT CAT TGG GCG TCC TC
|
RT-PCR |
| Sequence-based reagent |
Mouse Nox4 reverse |
IDT |
AGA ACT GGG TCC ACA GCA GA
|
RT-PCR |
| Sequence-based reagent |
Mouse β-actin forward |
IDT |
AGT GTG ACG TTG ACA TCC GT
|
RT-PCR |
| Sequence-based reagent |
Mouse β-actin reverse |
IDT |
TGC TAG GAG CCA GAG CAG TA
|
RT-PCR |
