Video 1. Wild-type zebrafish melanin.
This video shows an overview of melanin staining with silver by X-ray microtomography (micro-CT) imaging in a representative wild-type 5 days post-fertilization (dpf) zebrafish larva. The three-dimensional (3D) volume is generated from a series of 0.52 µm slices and reproduces pigmentation patterns seen by light microscopy (colored arrowheads). In a view of the top layer of the dorsal melanin stripe (false colored for clarity), ovoid transparencies are observed representing large organelles lacking melanin (circles). An isolated volume of the retinal pigment epithelia (RPE) exhibits the fused choroidal fissure and the optic nerve egress. Finally, a 360° view of the larval melanin, colored by anatomic region, is shown. Background music by DJ Vanselow.