Supplementary Figure 1.

Comparison of gestational age and plasma kisspeptin levels by Crown-Rump Length and Last Menstrual Period. A. Scatterplot of gestational age as estimated by Crown-Rump Length (CRL) (in days) over gestational age as estimated by Last Menstrual Period (LMP) (in days) in healthy controls (in grey) (n=101 women) and women with miscarriage (in red) (n=81 women). Data was analysed by simple linear regression (r²=0.82 in controls and r²=0.54 in miscarriage); ∗∗∗∗P<0.0001. B, C. Spaghetti plots of plasma kisspeptin with gestational age (in weeks) as estimated by Crown-Rump Length (CRL) (B) and Last Menstrual Period (LMP) (C). Red bars are 95% confidence limits for a mixed-effects linear regression model fitted to the data; blue bars are 95% prediction limits for the same model. Intraclass Correlation Coefficients (ICC) are calculated from the random effects variance for the models. ICC for panel B is 19.9% and ICC for panel C is 30.3%, indicating that 20% - 30% of the variation in kisspeptin can be explained by subject difference.