Supplementary Figure 2.

Classification tree presenting the risk of miscarriage on the basis of gestation-adjusted i.e. multiple of median (MoM) of kisspeptin and βhCG levels. The number of samples and the proportion that are from women with control pregnancies, or from women affected by miscarriage is presented in the box. From the ‘start’, the risk of miscarriage can be estimated by following the arrows according to the MoM of plasma kisspeptin, for which the categories are shown in blue. The risk of miscarriage is first categorised according to MoM kisspeptin levels; for example 68.7% of samples with a low MoM kisspeptin level of <0.12 were from women who suffered miscarriage, whereas only 1.5% were if MoM kisspeptin > 1.0. Women with MoM kisspeptin levels between 0.12 and 1.0 were further risk-stratified using MoM βhCG levels (red). The area under receiver operating characteristic curve (auROC) for correct categorization by this decision tree is 0.90 (95% CI: 0.87 – 0.93). MoM, Multiple of gestation-specific median values of women with healthy pregnancies; βhCG, beta chorionic gonadotropin (iU/L).