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. Author manuscript; available in PMC: 2021 Sep 16.
Published in final edited form as: FASEB J. 2019 Nov 29;34(1):1136–1149. doi: 10.1096/fj.201901657RR

FIGURE 7.

FIGURE 7

PIP5K1C binds PDZD7 long isoform and localizes in the stereocilia. A and B, HEK293T cells were transfected with the constructs indicated on the top of each panel. Immunoprecipitations were performed with immobilized Myc-antibody followed by western blotting to detect corresponding proteins. C, HeLa cells were transfected to express Myc-tagged PIP5K1C and mCherry-tagged PDZD7 long isoform. Immunostaining with anti-PIP5K1C antibody was performed to locate PIP5K1C. DAPI staining was performed to indicate nuclei. D, Cochlear explants from wild-type or Pdzd7ΔPDZ3/ΔPDZ3 mice at P3 were injectoporated with expression vectors to express HA-tagged PIP5K1C in hair cells. GFP fluorescence indicated successfully injectoporated hair cells. Immunostaining with anti-HA antibody was performed to locate HA-PIP5K1C. Phalloidin staining was performed to indicate the stereocilia. Scale bars: 5 μm in (C), 20 μm in (D)