Figure 1.

The quality and freshness of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are decreased over time after detachment from the culture dish. To evaluate the freshness and quality of hBM-MSCs, the metabolome is analyzed using gas chromatography–mass spectrometry (GC/MS), the transcriptome is analyzed using microarray, deformability is analyzed using microfluidics, and membrane fluidity is tested using differential interference contrast- (DIC-) total internal reflection fluorescence microscopy (TIRFM) in combination based on previous reports [9, 24].