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PIH inhibited Erastin-induced iron accumulation, MDA level, and ROS production in PC12. (a) Total iron concentration was detected by iron colorimetric assay kit (F = 30.98). (b, c) Cellular ROS levels were evaluated by flow cytometry using DCFH-DA probe (F = 555.6). (d) MDA activity was measured with lipid peroxidation MDA assay kit (F = 59.61, n =3). The data are presented as means ± SD. ∗∗P < 0.01, ∗∗∗P < 0.001; ns: not significant.
