Table 2.
Differential miRNA expression of a priori miRNAs by fire type exposure: separate models adjusted for age, BMI, ethnicity, batch effects.
| miRNA | Incumbents vs new recruitsa | New recruits (follow-up vs baseline measurement)b | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Employment duration | Fire-hours | Fire-runs | Most recent firec | ||||||||||||
| All fires | FC | 95% CI | FC | 95% CI | FC | 95% CI | FC | 95% CI | FC | 95% CI | |||||
| hsa-miR-1260a | 0.55 | 0.43 | 0.71 | 0.96 | 0.93 | 1.00 | 0.98 | 0.94 | 1.01 | 0.95 | 0.89 | 1.01 | 0.86 | 0.69 | 1.07 |
| hsa-miR-548h-5p | 0.59 | 0.51 | 0.69 | 1.05 | 1.02 | 1.08 | 1.04 | 1.02 | 1.07 | 1.09 | 1.04 | 1.14 | 1.02 | 0.83 | 1.26 |
| hsa-miR-145-5p | 0.44 | 0.32 | 0.61 | 0.96 | 0.93 | 0.98 | 0.97 | 0.94 | 0.99 | 0.94 | 0.90 | 0.98 | 0.83 | 0.72 | 0.96 |
| hsa-miR-4516 | 0.56 | 0.48 | 0.65 | 0.97 | 0.95 | 0.99 | 0.98 | 0.96 | 1.00 | 0.96 | 0.93 | 1.00 | 0.92 | 0.82 | 1.03 |
| hsa-miR-331-3p | 0.60 | 0.52 | 0.70 | 0.97 | 0.94 | 0.99 | 0.98 | 0.96 | 1.00 | 0.96 | 0.92 | 1.00 | 0.84 | 0.70 | 1.01 |
| hsa-miR-181a-5p | 0.62 | 0.53 | 0.72 | 0.99 | 0.97 | 1.02 | 0.99 | 0.97 | 1.02 | 0.99 | 0.95 | 1.03 | 0.81 | 0.70 | 0.92 |
| has-miR-5010-3p | 1.59 | 1.41 | 1.81 | 1.13 | 1.10 | 1.16 | 1.10 | 1.08 | 1.31 | 1.20 | 1.15 | 1.26 | 1.44 | 1.14 | 1.82 |
| hsa-miR-374a-5p | 1.72 | 1.40 | 2.13 | 1.03 | 0.99 | 1.07 | 1.03 | 1.00 | 1.06 | 1.05 | 0.99 | 1.11 | 1.09 | 0.89 | 1.34 |
| hsa-miR-486-3p | 3.35 | 2.59 | 4.33 | 1.15 | 1.10 | 1.21 | 1.12 | 1.07 | 1.16 | 1.23 | 1.14 | 1.32 | 2.20 | 1.66 | 2.91 |
| Structure fires | |||||||||||||||
| hsa-miR-1260a | 0.55 | 0.43 | 0.71 | 0.96 | 0.93 | 1.00 | 0.94 | 0.83 | 1.05 | 0.94 | 0.85 | 1.04 | 1.00 | 0.81 | 1.25 |
| hsa-miR-548h-5p | 0.59 | 0.51 | 0.69 | 1.05 | 1.02 | 1.08 | 1.17 | 1.08 | 1.28 | 1.13 | 1.05 | 1.22 | 1.12 | 0.94 | 1.35 |
| hsa-miR-145-5p | 0.44 | 0.32 | 0.61 | 0.96 | 0.93 | 0.98 | 0.93 | 0.86 | 1.01 | 0.94 | 0.88 | 1.00 | 0.82 | 0.66 | 1.00 |
| hsa-miR-4516 | 0.56 | 0.48 | 0.65 | 0.97 | 0.95 | 0.99 | 0.94 | 0.89 | 1.00 | 0.95 | 0.90 | 1.00 | 0.90 | 0.77 | 1.06 |
| hsa-miR-331-3p | 0.60 | 0.52 | 0.70 | 0.97 | 0.94 | 0.99 | 0.94 | 0.87 | 1.01 | 0.95 | 0.89 | 1.01 | 0.83 | 0.70 | 0.99 |
| hsa-miR-181a-5p | 0.62 | 0.53 | 0.72 | 0.99 | 0.97 | 1.02 | 1.01 | 0.94 | 1.09 | 1.00 | 0.94 | 1.07 | 0.74 | 0.61 | 0.90 |
| has-miR-5010-3p | 1.59 | 1.41 | 1.81 | 1.13 | 1.10 | 1.16 | 1.35 | 1.24 | 1.48 | 1.30 | 1.20 | 1.39 | 1.37 | 1.07 | 1.77 |
| hsa-miR-374a-5p | 1.72 | 1.40 | 2.13 | 1.03 | 0.99 | 1.07 | 1.07 | 0.97 | 1.20 | 1.07 | 0.97 | 1.16 | 1.10 | 0.89 | 1.35 |
| hsa-miR-486-3p | 3.35 | 2.59 | 4.33 | 1.15 | 1.10 | 1.21 | 1.38 | 1.21 | 1.60 | 1.35 | 1.20 | 1.52 | 1.81 | 1.33 | 2.45 |
Associations significant after Bonferroni correction are in bolded text.
FC fold-change, CI confidence interval.
aA priori markers were identified and originally presented in Jeong et al.[16].
bModels also adjusted for age, BMI, ethnicity, batch effects, and Bonferroni correction. For employment duration, effect is for a 6-month increase. For fire-hours, effect is for a 10-h increase. For fire-runs, effect is for a 10-fire increase.
cThe best measure of time since most recent fire exposure (continuous log-days, split at median, split at tertiles, or split at quantiles) was selected for each miRNA by the highest Akaike information criteria (AIC) value. For time since most recent fire exposure, effect is the earliest exposure compared to later exposure(s). hsa-miR-548h-5p and hsa-miR-374a-5p used time split at the median. Hsa-miR-1260a, hsa-miR-145-5p, hsa-miR-4516, hsa-miR-331-3p, hsa-miR-181a-5p, hsa-miR-5010-5p, and hsa-miR-486-3p used time split at quantiles.