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. 2021 Jul 27;40(37):5613–5625. doi: 10.1038/s41388-021-01961-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Lentivirus-mediated stable B-Myb overexpression. HCT116 and RKO cells were infected with the empty control and B-Myb-expressing lentiviral particles, and then selected in the presence of puromycin to obtain the control (LV-control) and B-Myb overexpression (LV-B-Myb) stable cells. Expression of B-Myb was examined by qRT-PCR and immunoblot analysis. b Lentivirus-mediated stable B-Myb knockdown. HCT116 and RKO cells were infected with the lentiviral particles expressing negative control shRNA and B-Myb shRNA, and then selected in the presence of puromycin to generate the polyclonal control (shNC) and B-Myb knockdown (shB-Myb) stable cells. Expression of B-Myb was examined by qRT-PCR and immunoblot analysis. c, d B-Myb increases cell proliferation. Cell proliferation was detected by CCK8 assay in the stable B-Myb overexpression or knockdown cells at the indicated time points. e, f B-Myb increases colony formation. Cells were seeded on plastic plates for plate clone formation assay. Representative images were shown. g, h B-Myb promotes colorectal cancer growth in vivo. Stable B-Myb overexpression or knockdown HCT116 cells were injected subcutaneously into the dorsal flanks of nude mice. The tumor size was measured 1–2 times a week for tumor growth curve construction. The tumor weight was measured at the end of the experiment. Data represent the mean ± SD. All experiments were performed in triplicates. *p < 0.05, **p < 0.005, ***p < 0.001.