Figure 1.

Ghrelin attenuates LPS-induced lung pathological alterations. Histopathological changes of lungs determined by H&E staining (×200). Male Balb/c mice were randomized into control, ghrelin, LPS, and ghrelin + LPS groups (n = 6, each group analyzed in triplicate). Lung histological changes were assessed 24 h after LPS instillation as described in Experimental procedures. A, representative images of H&E-stained sections of lung tissues for control, ghrelin, LPS, and ghrelin + LPS group. B, pathological scores of lung samples in each group are shown, indicating that treatment with ghrelin significantly ameliorates LPS-induced lung edema, hemorrhage, alveolar collapse, and inflammatory cell infiltration. Three slices of each mouse were used, and the observer randomly observed and photographed six fields of view. The observers were blinded to each experimental group. (n = 6, ∗p < 0.05 compared with the control group, ^#p < 0.05 compared with the LPS group). LPS, lipopolysaccharide.