Figure 2.

Effect of LPS and ghrelin on cell viability.A and B, viability of EA.hy 926 cells was estimated using the Cell Counting Kit-8 assay. Cells were cultured in DMEM with different concentration of LPS, with a median inhibitory concentration (IC₅₀) value of 150 μg/ml for 24 h. Later, cells were stimulated with 150 μg/ml LPS alone or in the presence of the indicated concentrations of ghrelin. According to the result, ghrelin at 100 nM plays a significant role on cell viability. Representative of three independent experiments, mean ± SEM, ∗p < 0.05 versus control. DMEM, Dulbecco’s modified Eagle’s medium; LPS, lipopolysaccharide.