Table 2.
Tm and ΔH values of RBD variants obtained from thermal denaturation curves using far-UV CD
| RBD variant | Tm (°C) | ΔTm (°C) | ΔH (kcal/mol) |
|---|---|---|---|
| Wildtype | 56.1 ± 0.7 | — | −1.7 ± 0.3 |
| K417N | 56.9 ± 0.3 | 0.8 ± 0.8 | −3.0 ± 0.4 |
| Y453F | 54.8 ± 0.4 | −1.3 ± 0.8 | −2.2 ± 0.3 |
| S477N | 55.8 ± 0.3 | −0.3 ± 0.8 | −1.9 ± 0.1 |
| T478I | 52.3 ± 0.2 | −3.8 ± 0.7 | −2.9 ± 0.3 |
| E484K | 52.3 ± 0.5 | −3.8 ± 0.9 | −2.0 ± 0.3 |
| S494P | 55.3 ± 0.4 | −0.8 ± 0.8 | −2.3 ± 0.3 |
| N501Y | 55.1 ± 0.3 | −1 ± 0.8 | −2.1 ± 0.1 |
| K417T/E484K/N501Y | 56.5 ± 0.2 | 0.4 ± 0.7 | −2.4 ± 0.2 |
Errors on ΔTm were calculated using error propagation formulae (74).