Table 4.
Interaction parameters obtained from binding of RBD variants to CC12.1 ScFv probed by ITC
| RBD variant | Kd (nM) | N | ΔH (kcal/mol) | ΔG (kcal/mol) | −TΔS (kcal/mol) |
|---|---|---|---|---|---|
| Wildtype | 28.0 ± 8.8 | 0.9 ± 0.0 | −4.8 ± 0.1 | −10.1 ± 0.2 | −5.3 ± 0.2 |
| K417N | 119 ± 50 | 0.8 ± 0.0 | −1.8 ± 0.1 | −9.3 ± 0.2 | −7.5 ± 0.3 |
| Y453F | 827 ± 146 | 1.0 ± 0.0 | −2.2 ± 0.1 | −8.2 ± 0.1 | −6.0 ± 0.1 |
| S477N | 25.6 ± 6.8 | 0.8 ± 0.0 | −5.7 ± 0.1 | −10.2 ± 0.2 | −4.5 ± 0.2 |
| T478I | 5.8 ± 3.4 | 0.8 ± 0.0 | −5.6 ± 0.1 | −11.0 ± 0.3 | −5.4 ± 0.4 |
| E484K | 1.7 ± 4.6 | 0.7 ± 0.0 | −5.5 ± 0.1 | −11.8 ± 1.6 | −6.2 ± 1.2 |
| S494P | 23.0 ± 7.7 | 0.8 ± 0.0 | −6.4 ± 0.1 | −10.2 ± 0.2 | −3.9 ± 0.2 |
| N501Y | 63 ± 22 | 0.9 ± 0.0 | −6.2 ± 0.2 | −9.7 ± 0.2 | −3.4 ± 0.3 |
| K417T/E484K/N501Y | 433 ± 95 | 1.0 ± 0.0 | −5.6 ± 0.2 | −8.5 ± 0.3 | −3.0 ± 0.2 |
Errors on ΔG and −TΔS were calculated using error propagation formulae (74).