Table 5.
Comparison of protein expression, stability, binding to ACE2, and binding to CC12.1 of RBD variants
| RBD variant | Expression | Tm (°C) |
Kd (nM) |
Kd (nM) |
|---|---|---|---|---|
| (ACE2 binding) | (CC12.1 binding) | |||
| Wildtype | — | 56.1 ± 0.7 | 10.0 ± 3.1 | 28.0 ± 8.8 |
| K417N | No change | 56.9 ± 0.3 | 94.1 ± 9.5 | 119 ± 50 |
| Y453F | Increase | 54.8 ± 0.4 | 11.4 ± 1.7 | 827 ± 146 |
| S477N | Increase | 55.8 ± 0.3 | 5.0 ± 1.4 | 25.6 ± 6.8 |
| T478I | Decrease | 52.3 ± 0.2 | 16.7 ± 5.6 | 5.8 ± 3.4 |
| E484K | Decrease | 52.3 ± 0.5 | 51.5 ± 8.2 | 1.7 ± 4.6 |
| S494P | Increase | 55.3 ± 0.4 | 13.8 ± 3.5 | 23.0 ± 7.7 |
| N501Y | No change | 55.1 ± 0.3 | 3.0 ± 2.1 | 63 ± 22 |
| K417T/E484K/N501Y | Increase | 56.5 ± 0.2 | 1.6 ± 1.5 | 433 ± 95 |
Bold values indicate parameters favoring the natural selection of variants.