Fig. 3.

FGF21 knockdown induces oxidative stress and worsen iron overload induced ferroptosis.

Fgf21 was knocked down in primary hepatocytes using Fgf21 siRNA, followed by treatment with or without FAC (1000 μM) for 24 h. (A) qRT-PCR analysis of Fgf21 mRNA expression in primary hepatocytes treated with control siRNA or Fgf21 siRNA. (B to E) Cell viability, LIP, ROS and CD11-BODIPY levels in the cells (n = 4). (F) Iron metabolism and ferroptosis related proteins levels in the cells (n = 3). (G–I) Cell viability, C11-BODIPY levels, and iron metabolism and ferroptosis related proteins expression in above primary hepatocytes treated with vehicle (mock) or 1000uM FAC.