Skip to main content
. 2021 Jun 1;12(4):1179–1199. doi: 10.1016/j.jcmgh.2021.05.018

Figure 5.

Figure 5

Kyn-mediated AhR activation promoted FoxP3 transcription. (A) Jurkat cells were treated with different CM_ or Kyn (500 μM) for 24 hours, and the messenger RNA level of FoxP3 was detected by real time PCR (n = 9). (B) AhR siRNA-transfected or CH-223191 (10 μM) (CH)–pretreated Jurkat cells were stimulated with different CM_, and the FoxP3 messenger RNA level was determined by real-time PCR (n = 9). (C–E) AhR siRNA-transfected Jurkat cells were stimulated with CM for 24 hours and FoxP3 expression was detected by Western blot (n = 3). (F, G) Mice spleen lymphocytes were cultured in the supernatant of colon cancer cells and CH-223191 (10 μM) for 24 hours (n = 3). The percentage of Tregs was measured. (H, I) ChIP assay was performed to illustrate the interaction between AhR and FoxP3 promoter (n = 3). The results were performed at least in triplicate and expressed as the mean ± SD. ∗P < .05, ∗∗P < .01 compared with the control, #P < .05, ##P < .01 compared with CM_ (A, B, D, E, G). ∗P < .05, ∗∗P < .01 indicates significant difference (H, I).∗P < .05, ∗∗P < .01 indicates significant difference.