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. 2021 Jun 1;12(4):1179–1199. doi: 10.1016/j.jcmgh.2021.05.018

Figure 8.

Figure 8

Absence of IDO contributed to increased CD8+T infiltration in colon. (A) Absolute numbers of CD4+ and CD8+ T cells infiltrated in tumor were measured by flow cytometry (n = 6). (B) The percentage of CD4+ and CD8+ T cells in spleen lymphocytes was analyzed by flow cytometry (n = 6). (C, D) Absolute numbers of CD4+ and CD8+ T cells in spleen lymphocytes were detected by flow cytometry (n = 6). (E, F) The frequency of intratumoral CD8+/ CD69+ T cells was determined (n = 6). (G, H) The percentage of CD8+ GZB+ T cells was detected (n = 6). (I, J) The expression of Ki67 and its mean fluorescence intensity (MFI) of CD8+ T were shown (n = 6). (K, L) Annexin V expression on intratumoral CD8+ T cells and its mean fluorescence intensity were determined by flow cytometry (n = 6). (M, N) Serial sections of colon tissues were immunostained with DAPI (blue), anti F4/80 (green), and CD8 (red). They were observed by confocal laser scanning microscope (scale bar = 50 μm, 10 μm). Expressions of CD11b (red) and CD4 (green) were analyzed by immunofluorescence cytochemistry (scale bar = 50 μm, 10 μm). The results are representative of at least 6 independent experiments and expressed as mean ± SD. ∗P < .05, ∗∗P < .01 indicates significant difference.