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. 2021 Sep 16;12:5473. doi: 10.1038/s41467-021-25383-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Allyl sulfide, 1,3-Dimercaptopropane, and photoinitiator DMPA are dissolved and mixed in chloroform. Upon activation with UV light polymerization generates PAM. b FT-IR analysis of PAM polymer and PAM polymer after oxidation with 10% H₂O₂, which generates sulfone groups. c ¹H-NMR of PAM and PAM after conjugation of tetra-PEG-SH via UV activated thiol-ene chemistry. Allyl protons are consumed in the reaction with PEG-SH. d PEG-PAM at 4 weight % self assembles to form a gel, G’ >G”. Rheometer measurement of modulus of 4% of tetra-PEG-SH, PEG-PAM, PEG-PAM after oxidation in PBS. Data is plotted showing the mean and Standard Deviation. Biological replicates derived from independent experiments are shown as a dot (n = 2).