Figure 3.

(A) Cell adhesion strength of HeLa Fucci cells on PLL-g-PEG-RGD surface as a function of cell color and morphology measured with the computer-controlled micropipette. Aggregated cell adhesion data shown in Fig. 2A were separated on the basis of cell morphology. As expected, round cells showed a lower affinity to adhere to the surface than flat cells did, regardless of cell color. (Flattened colorless cells were very rare, thus we could not collect enough data to obtain their adhesion curve.) (B) Determination of the average cell area as a function of cell color and cell morphology. All charts show results from 4 different micropipette experiments, and n = 31 flattened red cells, n = 57 rounded red cells, n = 56 flattened green cells, n = 34 rounded green cells, n = 57 flattened yellow cells, n = 38 rounded yellow cells, n = 32 rounded colorless cells. (C) Cells were divided into eight groups based on their color and morphology. From top to bottom: rounded red cell, flattened red cell, rounded green cell, flattened green cell, rounded yellow cell, flattened yellow cell, rounded colorless cell. Scale bars represent 10 µm.