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. 2021 Sep 16;11:18501. doi: 10.1038/s41598-021-97984-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transfection of fully mature schizonts of P. falciparum. (A) The fully mature schizonts enclosed within a single membrane of the parasitophorous vacuole (upper) or erythrocyte (lower) are indicated by arrows. (B). Purified schizonts, including fully mature schizonts, were transfected with 5 μg (closed circle/solid line) and 50 μg (opened circle/dashed line) of pFCENv1 plasmid. Five micrograms of pFCENv1 was also preloaded into RBCs and then introduced into the parasite by uptake (closed triangle/dashed line).