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. 2021 Sep 16;12:5479. doi: 10.1038/s41467-021-25748-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Bacterial population sizes in the ΔxopC2-inoculated leaves of the wild-type, IE-17, IE-D391A-2, and IE-N396A-14 transgenic seedlings. The 3-week-old seedlings were treated with mock and DEX followed by spraying with Xoc ΔxopC2 after 24 h. Bacterial populations were determined at 4 days post-inoculation (dpi). Data are shown as means ± standard error (SE) (n = 3 technical replicates per measurement). The letters (a, b) indicate a statistically significant difference in bacterial population sizes as revealed by one-way ANOVA, Tukey’s honest significance test. b Stomatal conductance in the leaves of the wild-type, IE-17, IE-D391A-2, and IE-N396A-14 transgenic lines after challenging with ΔxopC2. The 3-week-old seedlings were pretreated with DEX and mock followed by spray inoculation with ΔxopC2 and mock control. Stomatal conductance was measured at 2 dpi. Data are shown as means ± SE (n = 8 technical replicates per measurement). These experiments were independently repeated 3 times with similar results in a and b. The letters (a, b) indicate a statistically significant difference in stomatal conductance of the xopC2 transgenic IE-17 seedlings between mock and DEX treatments as revealed by one-way ANOVA, Tukey’s honest significance test.