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. 2021 Sep 16;12:5481. doi: 10.1038/s41467-021-25824-9

Fig. 8. FACS and sorted populations sequencing analyses.

Fig. 8

Gates P1, 14, and gate P16 define regions containing events positive and negative for the BDE525 fluorescent signal, respectively. A Relative abundance of gram-positive bacteria in the P1, P14, and P16 samples, and in total bacteria., determined from 16S rRNA gene community sequencing. Relative abundance was calculated by summing the abundance of reads assigned to the Firmicutes and Actinobacteria phyla, which contain the vast majority of gram-positive bacterial species divided by the total read number. Error bars show standard deviation Total DNA: 4 independent samples, in two technical replicates; All three gates were sorted twice, from three independent samples. Dots represent individual measurements. B Principal coordinate analysis of the sorted cells from the different gates. C Community composition analysis of the samples collected from gates P1, P14 and P16. Taxa are represented at the family level. D MRPP comparisons of Bray–Curtis distances between the communities in the sorted gates (P1, P14, P16) and the general community (total DNA). ns: 5.00e-02 < p < = 1.00e + 00; *: 1.00e-02 < p < = 5.00e-02, **: 1.00e-03 < p < = 1.00e-02; ***: 1.00e-04 < p < = 1.00e-03; ****: p < = 1.00e-04; no adjustment for multiple comparisons.