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. 2021 Sep 16;12:5488. doi: 10.1038/s41467-021-25809-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Fertility assay of 20-day-old females of the indicated genotypes. Results of four biological replicates are expressed as mean ± SD, **P < 0.01 by unpaired two-tailed t-test (n = 6 females/genotype/replicate). b IF analysis of wild-type (ctrl, upper right panel), Hecw^CI (CI, left panels) and Hecw^KO (KO, right panels) egg chambers. Mutants show aberrant number of nurse cells (top panels), compound egg chambers (middle panels), oocyte misspecification and apoptotic egg chambers (bottom panels). Blue, DAPI; red, phalloidin; white, Orb; asterisks indicate oocytes in compound egg chambers; white arrow indicates an Orb-positive nurse cell. Scale bar: 25 µm. c–e IB and IF analysis of the indicated genotypes. Hecw depletion in the germline causes oogenesis defects, as in (b), upper panel. Example of compound egg chamber in a 3-day-old Hecw-depleted fly (nos>RNAi Hecw) (c). The oogenesis defects are rescued in the Hecw^KO:DC504 genetic background (d). Ectopic expression of Hecw in the germline (nos>RFP-Hecw) induces oogenesis defects (e). Blue, DAPI; red, phalloidin. Asterisks indicate oocytes of the compound egg chamber. Scale bar: 25 µm. See also Supplementary Table 1 for the quantification.