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. 2021 Sep 16;12:5488. doi: 10.1038/s41467-021-25809-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a IF analysis of egg chambers from 3-day-old flies of the indicated genotypes with anti-Orb antibody (n = 3). White arrows indicate ectopic Orb puncta in nurse cells of Hecw^KO flies. b IF analysis of the indicated genotypes (n = 3). Green, Me31B::GFP which marks the RNPs; red, Orb which marks the oocyte (in white in the first panel); blue, DAPI; white, phalloidin. Scale bar: 25 μm. c Quantification of RNPs size of egg chambers (stages from 6–9) from 3-day-old flies of the indicated genotypes. Results are expressed as mean ± SD. n = 477 (ctrl Me31B::GFP), n = 559 (Hecw^KO;;Me31B::GFP), n = 444 (Hecw^CI;;Me31B::GFP), 5 egg chambers/genotype from two biological replicates. ****P < 0,0001 by two-tailed Mann–Whitney test. d IF analysis of egg chambers of 3-day-old flies as indicated (n = 3), not treated (nt) or treated with 1,6 hexanediol (1,6 hex). Scale bar: 50 μm. e Quantification of RNPs size in egg chambers (stage 7–9) of the indicated genotypes and conditions. Treatment significantly reduces RNP size in control but not in Hecw^KO and Hecw^CI flies (KO;Me31B::GFP and CI;Me31B::GFP). Particles analysed: n = 505 ctrl (nt), n = 49 ctrl (1,6 hex), n = 642 Hecw^KO (nt), n = 536 Hecw^KO (1,6 hex), n = 314 Hecw^CI (nt), n = 553 Hecw^CI (1,6 hex) (3 egg chambers/genotype). Size mean ± SD is reported. ****P < 0.0001 by two-tailed Mann–Whitney test.