Fig. 5. Lack of Hecw does not alter RNPs mobility.

a Representative intermediate scattering functions $f\left(q,\Delta t\right)$ obtained from Differential Dynamic Microscopy (DDM) analysis of an image sequence of a KO;Me31B::GFP egg chamber plotted as a function of the time delay $△t$ for different values of the wave vector $q$ in the range $0.4-0.8\mathrm{\mu }{\mathrm{m}}^{-1}$.  b From each of the curves shown in (a), a $q$-dependent estimate of the mean square displacement $MS{D}_q\left(\mathrm{\Delta }t\right)\equiv -\frac{4}{q^2}\ln f\left(q,\mathrm{\Delta }t\right)$ can be obtained (coloured symbols). Black circles correspond to the best estimate for the average mean square displacement $MS{D}_0\left(\mathrm{\Delta }t\right)$ of the RNPs, obtained by extrapolating $MS{D}_q\left(\mathrm{\Delta }t\right)$ to $q=0$ at fixed $\mathrm{\Delta }t$. The extrapolation procedure is illustrated in the inset for the representative case $\mathrm{\Delta }t=10s.$ c Average mean square displacement $MS{D}_0\left(\mathrm{\Delta }t\right)$ (symbols) of the RNPs in the considered egg chamber, displaying a characteristic linear dependence on the delay time $\mathrm{\Delta }t$, which is indicative of a random, diffusive-like motion. An effective diffusion coefficient $D_0$ can be obtained by fitting a linear model $MS{D}_0\left(\mathrm{\Delta }t\right)=4D_0\mathrm{\Delta }t$ to the data (continuous black line). d DDM analysis of N = 18 egg chambers shows no significant difference in the RNP mobility between wild-type (Me31B, $D_{0,WT}=0.023\pm 0.006\mathrm{\mu }{\mathrm{m}}^2/\mathrm{s}$ N = 9) and Hecw^KO egg chambers (KO;Me31B, $D_{0,KO}=0.021\pm 0.005\mathrm{\mu }{\mathrm{m}}^2/\mathrm{s}$, N = 9). See also Supplementary Fig. 5h, i.