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. 2021 Sep 16;11:18519. doi: 10.1038/s41598-021-97220-8

Figure 2.

Figure 2

DHF downregulates expression of HIV-1 chemokine co-receptors CXCR4 and CCR5. Legend: CXCR-4 and CCR5 expression in the hippocampus and cortex of Wild Type (WT), Tg26, and DHF treated Tg26 mice (A-P). Immunohistochemical stained sections show CXCR-4 and CCR5 expressing cells in the hippocampal and cortex regions of the mice brains. 3 WT mice, 3 Tg26 mice, and 4 TG + DHF mice were used. A, B, C, E, F, G, I, J, K, M, N, and O are 400 × magnification pictures of the hippocampus and cortex regions of the mice. (D) Quantification of CXCR-4 expressing cells in the hippocampus: p < 0.001, WT versus Tg; p < 0.05 Tg versus Tg + DHF. (H) Quantification of CXCR-4 expressing cells in cortex: p < 0.001, WT versus Tg; p < 0.001, Tg versus Tg + DHF. (L) Quantification of CCR-5 expressing cells in the hippocampus: p < 0.001, WT versus Tg; p < 0.001 Tg versus Tg + DHF. (P) Quantification of CCR-5 expressing cells in cortex: p < 0.01, WT versus Tg; p < 0.001, Tg versus Tg + DHF. The total numbers of hippocampal fields analyzed (WT, Tg26, TG + DHF) for each antibody were CXCR-4 (63, 103, 92), CCR5 (110, 92, 71). The total numbers of cortex fields analyzed (WT, Tg26, TG + DHF) for each antibody were CXCR-4 (60, 60, 77), CCR5 (58, 59, 53). One Way ANOVA with Bonferroni's Multiple Comparison post- test. scale bar, 100 μm.