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. 2021 Sep 16;11:18519. doi: 10.1038/s41598-021-97220-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DHF treatment reduced astrogliosis in the brain of Tg26 mice: Legend: GFAP expression in the hippocampus and cortex of Wild Type (WT), Tg26, and DHF treated Tg26 mice (A-H). Immunohistochemical stained sections show GFAP expressing cells in the hippocampal and cortex regions of the mice brains. 3 WT mice, 3 Tg26 mice, and 4 TG + DHF mice were used. A, B, C and E,F,G are 400 × magnification pictures of the hippocampus and cortex regions, respectively, of the mice. (D) Quantification of GFAP expressing cells in the hippocampus: p < 0.001, WT versus Tg; p < 0.05 Tg versus Tg + DHF. (H) Quantification of GFAP expressing cells in cortex: p < 0.01, WT versus Tg; p < 0.05, Tg versus Tg + DHF. The total numbers of hippocampal fields analyzed (WT, Tg26, TG + DHF) were (61, 74, 107). The total numbers of cortex fields analyzed (WT, Tg26, TG + DHF) were (15, 15, 20). One Way ANOVA with Bonferroni's Multiple Comparison post-test. scale bar, 100 μm.