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. 2021 Sep 3;15:693095. doi: 10.3389/fncel.2021.693095

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Copyright © 2021 Malchow, Tchernookova, Choi, Smith, Kramer and Kreitzer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibitors of calmodulin, PKC, and carbonic anhydrase (CA) reduce the ATP-elicited increase in H⁺ flux from retinal Müller cells isolated from the retina of the tiger salamander. (A) 100 μM of the calmodulin inhibitor CLP potently inhibited the rise in H⁺ flux from an isolated Müller cell induced by 10 μM ATP. (B) Reduction of ATP-elicited increase in H⁺ flux from a single tiger salamander Müller cell induced by 10 μM ATP upon the addition of 10 μM of the PKC inhibitor chelerythrine. (C) Inhibition of H⁺ efflux from a single Müller cell by 1 mM of the carbonic anhydrase inhibitor acetazolamide. Asterisk represents a background control reading taken 200 μm above the cell.