Skip to main content
. 2021 Jun 22;27(10):1224–1237. doi: 10.1111/cns.13699

FIGURE 6.

FIGURE 6

AIM2 knockdown decreased neutrophil adhesion in vitro after OGD/R, and this effect was potentially associated with a decrease in p‐STAT3 expression in endothelial cells. A, The protein levels of STAT3, p‐STAT3, ICAM‐1, and GAPDH in HBMECs after OGD/R as determined by Western blot. B, C, Quantification of the levels of p‐STAT3 and ICAM‐1 normalized to that of GAPDH. D, The neutrophil purity was examined by flow cytometry. E, Representative images and quantification of neutrophil adhesion to HBMEC monolayers in vitro. Scale bar: 100 μm. F, Western blot images of STAT3, p‐STAT3, ICAM‐1, and GAPDH expression in PBMECs after OGD/R. G, H, Quantification of the levels of p‐STAT3 and ICAM‐1 normalized to that of GAPDH. All data are presented as the mean ±SEM. * p < 0.05 and ** p < 0.01 compared with the Lv‐control group. # p < 0.05 and ## p < 0.01 compared with the OGD/R‐Lv‐control group